anti dvl3 antibody  (Santa Cruz Biotechnology)

Journal: bioRxiv

Article Title: Dishevelled-mediated clustering stabilizes Frizzled6 and Vangl2 to establish planar cell polarity in the mammalian skin

doi: 10.64898/2026.05.01.722170

Figure Lengend Snippet: (A) Average intensity Z-stack projection of E15.5 wholemount backskins from control littermate ( Dvl2fl/fl; Dvl3+/+) and Dvl2/3 double mutant (K14-Cre; Dvl2fl/fl; Dvl3−/− ) embryos immunostained for P-cadherin and Sox9 to label anterior and posterior cell types of developing hair follicles, respectively. Scale bar: 200μm. Zoomed in view of individual follicles imaged at higher magnification shown to the right. Scale bar: 10μm. Anterior is to the left. (B) Plot depicting percentage of polarized follicles in littermate controls and Dvl2/3−/− double mutant skin (littermates color-matched). (C) Plot depicting density of germ-stage follicles in littermate controls and Dvl2/3 −/− double mutant skin (littermates color-matched). (D) Images show planar view of the basal layer of interfollicular epidermis, stained for Celsr1 (gray), Fz6 (green), Vangl2 (yellow) and E-cadherin (cyan). Images are overlaid with polarity nematics indicating the orientation (angle of the line) and magnitude (length of the line) of asymmetry. On the right, circular histograms depicting the angular distribution of polarity. (A-C) 722 follicles from n=4 control littermate embryos and 690 follicles from n=4 Dvl2/3−/− embryos. (D) Circular histograms constructed from: n= 7916 basal cells from 4 control embryos and n= 5498 basal cells from 4 Dvl2/3−/− embryos stained for Celsr1; n= 9364 basal cells from 4 control embryos and n=10,684 basal cells from 4 Dvl2/3−/− embryos stained for E-cadherin; n= 8968 basal cells from 4 control embryos and n= 9456 basal cells from 4 Dvl2/3−/− embryos stained for Fz6; n= 9586 basal cells from 4 control embryos and n=10360 basal cells from 4 Dvl2/3−/− embryos stained for Vangl2. Scale bar: 10μm

Article Snippet: The Dvl3 mutant 129S- Dvl3 tm1Awb /J (Strain #:009083)( ) and Dvl2flox mouse B6;129- Dvl2 tm1.1Wds /J, (Strain #:029061)( ) mice were obtained from Jackson laboratories.

Techniques: Control, Mutagenesis, Staining, Construct

Journal: bioRxiv

Article Title: Dishevelled-mediated clustering stabilizes Frizzled6 and Vangl2 to establish planar cell polarity in the mammalian skin

doi: 10.64898/2026.05.01.722170

Figure Lengend Snippet: (A) Schematic of Dvl3 protein domains. (B) Representative examples of keratinocytes transfected with Celsr1-Myc, Fz6-GFP and either full-length mScarlet3-Dvl3 (mSc-Dvl3) or deletion mutants Dvl3ΔDEP, Dvl3ΔDIX, Dvl3ΔIDR-C. (C) Junction enrichment ratios with median and inter-quartile range of full length and deletion variants of Dvl3. n= 75 junctions for Dvl3, 50 junctions for Dvl3ΔDEP, 88 junctions for Dvl3ΔDIX and 56 junctions of Dvl3ΔIDR-C, pooled from three replicates. (D) Schematic of the workflow of intra-uterine lentiviral transduction of E9.5 embryos to transduce the surface ectoderm for visualization of mSc-Dvl3 variants at E15.5. (E) Representative examples of epidermal basal layer from e15.5 embryos transduced with lentivirus carrying either full-length mSc-Dvl3, mSc-Dvl3ΔDEP, or mSc-Dvl3ΔDIX (magenta) and co-stained for Fz6 (green). White asterisks mark transduced cells in the basal layer. Merged insets with arrowheads indicate colocalization of mSc-Dvl3 with Fz6. (F) Pearson’s correlation coefficients between Fz6 and either mSc-Dvl3, mSc-Dvl3ΔDEP, or mSc-Dvl3ΔDIX as a measure of colocalization. Median and interquartile range are shown. n=88 cell clusters from 3 transduced embryos for mSc-Dvl3, n=93 cell clusters from 4 transduced embryos for mSc-Dvl3ΔDIX and n=29 cell clusters from 1 transduced embryo for mSc-Dvl3ΔDEP. ****: p<0.0001, tested by KS-test. scale bar: 10μm.

Article Snippet: The Dvl3 mutant 129S- Dvl3 tm1Awb /J (Strain #:009083)( ) and Dvl2flox mouse B6;129- Dvl2 tm1.1Wds /J, (Strain #:029061)( ) mice were obtained from Jackson laboratories.

Techniques: Transfection, Transduction, Staining

Journal: bioRxiv

Article Title: Dishevelled-mediated clustering stabilizes Frizzled6 and Vangl2 to establish planar cell polarity in the mammalian skin

doi: 10.64898/2026.05.01.722170

Figure Lengend Snippet: (A) Representative planar views of the epidermal basal layer at E13.5 and E15.5 labeled with anti-Dvl2/3 antibody (magenta). Right panels show segmented areas of Dvl2/3 accumulation into enriched domains/puncta. Arrowheads point to puncta of Dvl2/3 at E15.5. (B) Normalized frequency distribution of Dvl2/3 puncta sizes with each bar representing one replicate of E13.5 (light pink) and E15.5 (dark pink) epidermis from 4 and 3 embryos, respectively. The inset shows normalized frequency distribution of pooled puncta sizes. p<0.0001 by Chi-Square test. n= 1159 puncta/enriched domains from 4 embryos at E13.5 and 1304 puncta/enriched domains from 3 embryos at E15.5. (C) Percent junctional area covered by Dvl2/3 puncta/enriched domains at E13.5 and E15.5. Median and inter-quartile range are shown. n= 40 ROIs from 4 embryos at E13.5 and n=38 ROIs from 3 embryos at E15.5. (D) Representative planar view of E15.5 epidermal basal layer transduced with mSc-Dvl3 or mSc-Dvl3ΔDIX (magenta), and immunostained for Fz6 (green). Right panels and arrowheads show segmented puncta/enriched domains. (E) Quantification of normalized frequency distribution of mSc-Dvl3 and mSc-Dvl3ΔDIX puncta sizes. Note only mSc-Dvl3 forms large puncta. Each bar represents one replicate of 3 embryos transduced with mSc-Dvl3 (dark pink) and 4 embryos transduced with mSc-Dvl3ΔDIX. Inset shows normalized frequency distribution of puncta sizes pooled from 3 and 4 embryos transduced with mSc-Dvl3 and mSc3-Dvl3ΔDIX, respectively. n= 245 puncta from 89 transduced cell clusters or single cells across 3 embryos for mSc3-Dvl3; n= 34 puncta from 93 cell clusters or single cells across 4 embryos for mSc3-Dvl3ΔDIX. (F) Percent junctional area occupied by puncta/enriched domains of mSc-Dvl3 or mSc-Dvl3ΔDIX, with median and interquartile range. p<0.0001, KS test. (G) Puncta density (# detected puncta per unit junctional area) for mSc-Dvl3 and mSc-Dvl3ΔDIX, with median and interquartile range. p<0.0001, KS test. (H) Images of basal cells transduced with mSc-Dvl3 or mSc-Dvl3ΔDIX highlighting polarized accumulation of mSc-Dvl3 along posterior edges of cell clusters (white arrowheads). Transduced cells marked by asterisks in Fz6 channel. (I) Fluorescence intensity ratio of mSc-Dvl3 and mSc-Dvl3ΔDIX at posterior versus anterior junctions with median and inter-quartile range. n=91 cells for mSc-Dvl3 from 3 embryos and n=95 cells for mSc-Dvl3ΔDIX from 4 embryos. p<0.0001 by KS test. Scale bars: 10μm.

Article Snippet: The Dvl3 mutant 129S- Dvl3 tm1Awb /J (Strain #:009083)( ) and Dvl2flox mouse B6;129- Dvl2 tm1.1Wds /J, (Strain #:029061)( ) mice were obtained from Jackson laboratories.

Techniques: Labeling, Transduction, Fluorescence